Claudio A.T. Suazo
The problem of restoration in fluorescence microscopy deals at the
same time with a frequency cut off, blurring the image, and photon
noise, that corrupts the image by inserting elements that are not
present in the real object and also distorting the contrast. These
distortions hinder the possibility of using the images for
visualization, recognition and analysis using the three-dimensional
data. The algorithms developed to restore the lost frequencies and
perform band extrapolation, in general, assume absence of noise or a
white additive noise. This paper presents an approach to an improved
restoration through the band extrapolation of the images dealing with
the noise present in real images. An extrapolation algorithm using
constraints on both spatial and frequency domains with a smoothing
operator were combined with the Richardson-Lucy iterative algorithm.
The results of the method for simulated data are compared with the
ones obtained by the original Richardson-Lucy restoration algorithm
and with regularization by Total Variation. The extrapolation of
frequencies is also analysed both in synthetic and real images. The
method improved the results by obtaining higher signal-to-noise ratio
and quality index values, performing band extrapolation that allow a
better visualization of the three-dimensional structures..
Ponti-Jr. M, Mascarenhas N, Ferreira P, Suazo C (2012, to appear) Three-dimensional Noisy Images Restoration using Filtered Extrapolation and Deconvolution Signal, Image and Video Processing. DOI Link - PDF Preprint
Ponti-Jr M, Mascarenhas N, Suazo C (2007) A
restoration and extrapolation iterative method for band-limited
fluorescence microscopy images. In: IEEE
Proceedings of 20th Braz. Symp. Comp. Graph. and Image Processing,
pp. 271–280, SIBGRAPI 2007. DOI Link - PDF
Supplementary material
Obs: all images were adjusted to [0-255] gray levels for a better visualization|
Parameter |
PSF 1 |
PSF 2 |
PSF 3 |
|
Resolution (x, y) |
128 |
128 |
256 |
|
Axial resolution |
128 |
128 |
32 |
|
Lateral amplif. |
100x |
20x |
20x |
|
Num.Aperture |
1.3 |
0.75 |
0.45 |
|
Emission wavelength |
530nm |
530nm |
500nm |
|
Pixel resolution (x,y) |
0.14µm |
0.71µm |
0.100µm |
|
Distance z direction |
0.14µm |
0.71µm |
0.180µm |
|
Medium refraction index |
1.51 |
1.51 |
1.00 |
|
Specimen refraction index |
1.33 |
1.33 |
1.33 |
Last update Fev, 2012
